Targeting the Nucleosome Core Particle with Polyamide Dimers
نویسندگان
چکیده
The Nucleosome Core Particle (NCP) is the minimal unit in eukaryotic chromatin and a unique target for DNA-binding polyamides. The ability to modulate the stability of nucleosomes would be a useful tool for molecular biology and gene regulation. A series of polyamide clamps designed to differentiate between the NCP and B-form DNA were synthesized and analyzed using DNase I footprinting. In addition, a new polyamide dimer designed to bind to and stabilize the NCP was synthesized. Finally, the NCP was used to perform the DNA-templated ligation of alkyneand azide-functionalized polyamides.
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